Highly prevalent TERT promoter mutations in aggressive thyroid cancers
- Xiaoli Liu,
- Justin Bishop1,
- Yuan Shan2,
- Sara Pai3,
- Dingxie Liu,
- Avaniyapuram Kannan Murugan,
- Hui Sun4,
- Adel K El-Naggar5 and
- Mingzhao Xing
- Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, Johns Hopkins University School of Medicine, 1830 East Monument Street, Suite 333,
Baltimore, Maryland 21287, USA
1Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
2Departments of Anatomic Pathology and Neuro-Oncology, Moffitt Cancer Center, Tampa, Florida 33612, USA
3Department of Otolaryngology–Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
4Jilin Provincial Key Laboratory of Surgical Translational Medicine, Department of Thyroid and Parathyroid Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province 130033, China
5Department of Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
- (Correspondence should be addressed to M Xing; Email: mxing1{at}jhmi.edu)
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Figure 1Sequencing of the human TERT promoter electropherograms. Representative electropherograms of the genomic DAN sequencing of the human TERT promoter for the two indicated mutations are shown. (A) The sense DNA strand obtained using the sense primer for sequencing, displaying TERT promoter mutations C228T and C250T in various thyroid cancer cell lines and thyroid cancer samples. (B) The antisense DNA strand obtained using the antisense primer for sequencing, displaying TERT promoter mutations G228A and G250A in various thyroid cancer cell lines and thyroid cancer samples. (A and B) WRO cell line is used to show the wild-type human TERT promoter. PTC, papillary thyroid cancer; FTC, follicular thyroid cancer; ATC, anaplastic thyroid cancer; PDTC, poorly differentiated thyroid cancer.
- © 2013 Society for Endocrinology
