Role of estrogen receptor signaling in skeletal response to leptin in female ob/ob mice
- Russell T Turner1,2,
- Kenneth A Philbrick1,
- Amida F Kuah1,
- Adam J Branscum3 and
- Urszula T Iwaniec1,2⇑
- 1Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
- 2Center for Healthy Aging Research, Oregon State University, Corvallis, Oregon, USA
- 3Biostatistics Program, School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon, USA
- Correspondence should be addressed to U T Iwaniec; Email: urszula.iwaniec{at}oregonstate.edu
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Figure 1
Effects of 1 month of daily sc leptin injection (40 µg/mouse/day) in the presence or absence of the potent estrogen receptor antagonist ICI 182,780 (ICI) on body weight (A) and food intake (B) in female ob/ob mice. Data are mean ± s.e., n = 5–9/group. aDifferent from WT mice, P < 0.05. bDifferent from vehicle-treated ob/ob mice, P < 0.05.
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Figure 2
Effects of 1 month of daily sc leptin injection (40 µg/mouse/day) in the presence or absence of the potent estrogen receptor antagonist ICI 182,780 (ICI) on abdominal white adipose tissue (WAT) weight (A) and uterine weight (B) in female ob/ob mice. Data are mean ± s.e., n = 5–9/group. aLeptin + ICI treated ob/ob mice different from vehicle-treated WT mice, P < 0.05; bleptin + ICI treated ob/ob mice different from vehicle-treated ob/ob mice, P < 0.05; cleptin + ICI treated ob/ob mice different from leptin-treated ob/ob mice, P < 0.05.
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Figure 3
Effects of 1 month of daily sc leptin injection (40 µg/mouse/day) in the presence or absence of the potent estrogen receptor antagonist ICI 182,780 (ICI) on longitudinal bone growth rate (A), mineralizing perimeter (B), mineral apposition rate (C), bone formation rate (D), osteoblast perimeter (E), osteoclast perimeter (F), marrow adiposity (G), adipocyte density (H) and adipocyte size (I) in distal femur metaphysis in female ob/ob mice. Representative photomicrographs illustrating differences in fluorochrome labeling in WT mice treated with vehicle (J), ob/ob mice treated with vehicle (K), ob/ob mice treated with leptin (L) and ob/ob mice treated leptin and ICI (M). Data are mean ± s.e., n = 5–9/group. aLeptin + ICI treated ob/ob mice different from vehicle-treated WT mice, P < 0.05; bleptin + ICI treated ob/ob mice different from vehicle-treated ob/ob mice, P < 0.05; cleptin + ICI treated ob/ob mice different from leptin-treated ob/ob mice, P < 0.05. Scale bar, 50 µm.
- © 2017 Society for Endocrinology
