Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat
- Sjoerd D Joustra1,2⇑,
- Onno C Meijer2,
- Charlotte A Heinen3,4,
- Isabel M Mol2,
- El Houari Laghmani1,
- Rozemarijn M A Sengers1,
- Gabriela Carreno5,
- A S Paul van Trotsenburg3,
- Nienke R Biermasz2,
- Daniel J Bernard6,
- Jan M Wit1,
- Wilma Oostdijk1,
- Ans M M van Pelt7,
- Geert Hamer7 and
- Gerry T M Wagenaar1
- 1Department of Pediatrics, Leiden University Medical Center, Leiden, The Netherlands
2Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands
3Department of Pediatric Endocrinology, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands
4Endocrinology and Metabolism, Academic Medical Center, The Netherlands
5Developmental Biology and Cancer Programme, Institute of Child Health, London, UK
6Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada
7Center for Reproductive Medicine, Women's and Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands
- Correspondence should be addressed to S D Joustra; Email: s.d.joustra{at}lumc.nl
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Figure 2
IGSF1 immunohistochemical staining was observed in the rat brain at embryonic day 15, specifically in Rathke's pouch (A and B) and the choroid plexus (A and C). The lower boxed region in (A) is magnified in (B) and the upper boxed region is magnified in (C). In the adult rat, IGSF1 was present in the choroid plexus both at the protein (D) and mRNA levels (E). CP, choroid plexus; RP, Rathke's pouch; TV, third ventricle; LV, lateral ventricle; NC, notochord.
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Figure 3
In the adult rat, IGSF1 was present in the pituitary gland at the protein (A) and mRNA levels (B). Igsf1 mRNA was expressed mostly in the periventricular and medial parts of the adult rat hypothalamus (C), were it co-localized principally with GFAP (D). In the adult rat pituitary, IGSF1 protein co-localized mostly with PIT1 (E). TH, tyrosine hydroxylase; Gad67, glutamic acid decarboxylase 67; Vglut, vesicular glutamate transporter; GFAP, glial fibrillary acidic protein.
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Figure 5
IGSF1 immunohistochemical staining was observed in the abdomen of rat at embryonic day 15, mostly in the liver, adrenal gland, and pancreas (A, B, and C). The left boxed region in (A) is magnified in (B) and the right boxed region is magnified in (C). Expression in the liver was transient and disappeared the first days after birth (D), resulting in the absence of IGSF1 in adult liver at the protein (E). In the islets of Langerhans of the adult rat pancreas, IGSF1 was observed at the protein (F), but not at the mRNA level (G). In the adult rat adrenal gland, IGSF1 was confined to the zona glomerulosa of the cortex, both at the protein (H) and mRNA level (I). Lu, lung; A, adrenal; Li, liver; Ki, kidney; P, pancreas.
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Figure 6
Variable expression IGSF1 protein was observed in adult rat testis during representative epithelial stages II–III (A), IV and XII (B), and X (C). Testicular protein expression was corroborated by detection of Igsf1 mRNA (D), showing high expression in Sertoli cells in many, but not all, seminiferous tubules and little to no expression in Leydig cells. Arrow, Sertoli cells; S, spermatogonia; L, leptotene spermatocytes; Z, zygotene spermatocytes; P, pachytene spermatocytes; R, round spermatids; E, elongating spermatids; *, germ granules.
- © 2015 Society for Endocrinology
