Figure 7
Suppression effects by kinase inhibitors on CORT-induced spinogenesis. Kinase inhibitors were co-treated with 30 nM CORT on
isolated hippocampal slices. A 1 h treatment in ACSF without drugs (control), with 30 nM CORT (CORT), with 30 nM CORT and
10 μM H89 (PKA inhibitor) (CORT+H89), with 30 nM CORT and 10 μM chelerythrine (PKC inhibitor) (CORT+Chel), with 30 nM CORT
and 25 μM U0126 (ERK inhibitor) (CORT+U), with 30 nM CORT and 10 μM LIMK inhibitor (CORT+LIMKi), and with 30 nM CORT and 10 μM
SP600125 (JNK inhibitor) (CORT+SP). (A) Representative images of confocal micrographs; the spines along dendrite with CORT
and H89, with CORT and chelerythrine, with CORT and U0126, with CORT and LIMK inhibitor. Maximal intensity projections onto
XY plane from z-series confocal micrographs (MAX-XY), images analyzed by Spiso-3D (Spiso), and three-dimensional model illustrations
(model) are shown together. Bar, 5 μm. (B) Effects by the co-treatment of kinase inhibitors on the total spine density. Vertical
axis represents the average number of spines per 1 μm of dendrite. Data are represented as mean±s.e.m. (C) Histogram of spine head diameters after a 1 h treatment in ACSF without drugs (closed white circle), with CORT (closed
black circle), with CORT and H89 (closed red circle), with CORT and chelerythrine (closed blue circle), with CORT and U0126
(closed green circle), with CORT and LIMK inhibitor (closed orange circle), and with CORT and SP600125 (closed purple circle).
(D) Density of three subtypes of spines after a 1 h treatment in ACSF without drugs (white column), with CORT (black column),
with CORT and H89 (red column), with CORT and chelerythrine (blue column), with CORT and U0126 (green column), with CORT and
LIMK inhibitor (orange column), and with CORT and SP600125 (purple column). From left to right, small-head spines (small),
middle-head spines (middle), and large-head spines (large) type. Data are represented as mean±s.e.m. The statistical significance yielded: **P<0.01, *P<0.05 vs ‘CORT’. For each drug treatment, we investigated three to four rats, six to eight slices, 30–40 neurons, 60–80 dendrites,
and ∼3000–4000 spines. A full colour version of this figure is available at http://dx.doi.org/10.1530/JOE-15-0078.