UCP1 is present in porcine adipose tissue and is responsive to postnatal leptin
- Alison Mostyn1,
- Linda Attig,
- Thibaut Larcher2,
- Samir Dou,
- Pascale Chavatte-Palmer3,
- Monia Boukthir4,
- Arieh Gertler5,
- Jean Djiane6,
- Michael E Symonds7 and
- Latifa Abdennebi-Najar⇑
- UP 2012.10.101 EGEAL, Institut Polytechnique LaSalle, Beauvais, France
1School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UK
2INRA UMR 703, Ecole Nationale Vétérinaire, Nantes, France
3INRA, UMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, France
4Unité de Recherche 04UR08/03, Faculté de Médecine, Tunis, Tunisia
5The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
6Unité NOPA, INRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, France
7Early Life Research Unit, Academic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK
- Correspondence should be addressed to L Abdennebi-Najar; Email: latifa.najar{at}lasalle-beauvais.fr
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Figure 1
Effect of leptin administration once daily on body temperature in piglets with intrauterine growth restriction (IUGR). These were treated once a day with either saline (n=20, represented by open circles) or leptin (n=17, represented by filled circles) over the first 5 days of life and were also compared with untreated normal-sized piglets (n=24, represented by filled squares). Significant differences (P<0.05) between IUGR Sal and Control (represented by #) and IUGR lep (represented by *).
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Figure 2
Effect of leptin administration once daily over the first 5 days of life to piglets showing intrauterine growth restriction (IUGR) on the morphological appearance of perirenal adipose tissue sampled at 21 days of age from females. (A) Histological analysis of control (n=8) and IUGR piglets treated with either saline (IUGR Sal, n=4) or leptin (IUGR Lep, n=4). W and O indicate white and brown adipocytes respectively. (B) The mean area of brown (BAT) and white adipose tissues (WAT) and white adipocyte density. The values are means with their standard errors and significant differences from either IS or C indicated by *P<0.05.
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Figure 3
Effect of leptin administration once daily over the first 5 days of life to piglets showing intrauterine growth restriction (IUGR) on the presence of uncoupling protein 1 (UCP1) in perirenal adipose tissue sampled at 21 days of age from females. (A) Representative immunohistochemistry images from each group of piglets together with subcutaneous adipose tissue taken from an age-matched normal-sized animal and (B) Mean UCP1 abundance from 8 to 9 piglets per group as determined by western blot densitometry including representative blotting images (P, positive control (from a 1-day-old sheep); C, control; IS, IUGR administered saline, L, IUGR administered leptin); (C) Example image for perirenal adipose tissue sampled from a 21-day-old piglet (porcine IUGR Lep) and 7-day-old sheep (Ovine). The values are means with their standard errors and significant differences between groups indicated by *P<0.05.
- © 2014 Society for Endocrinology
