Downregulation of decidual SP1 and P300 is associated with severe preeclampsia
- Yachao Zhang1,2,3,*,
- Jieqiong Yang1,2,*,
- Shijian Lv1,2,
- Dong-Qin Zhao3,
- Zi-Jiang Chen1,2⇑,
- Wei-Ping Li1,2⇑ and
- Cong Zhang1,2,3⇑
- 1Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- 2Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, China
- 3Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Ji’nan, Shandong, China
- Correspondence should be addressed to C Zhang or Z-J Chen or W-P Li: zhangxinyunlife{at}163.com or chenzijiang{at}hotmail.com or liweiping{at}renji.com
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Figure 1
Levels of SP1 and P300 mRNA and protein in the decidual tissues. (A) SP1 and P300 mRNA levels in deciduae from NP (n = 21) and SPE (n = 21). (B) Expression of SP1 and P300 proteins relative to β-actin (n = 21 for each group). The data are shown as mean ± s.e.m. *P < 0.05. NP, normal pregnant; SPE, severe preeclampsia.
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Figure 2
The expression of SP1, P300, IGFBP1 and PRL in hESCs after induced decidualization for 6 days. (A, B, C, and D) Expression of SP1, P300, IGFBP1 and PRL mRNA relative to GAPDH respectively. (E and F) Levels of SP1 and P300 proteins. All data are shown as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001 vs Ctrl or day 0. ## P < 0.01 vs day 3. Ctrl, control hESCs; cAMP + MPA, treated hESCs. hESCs, human endometrial stromal cells. IGFBP1, insulin-like growth factor-binding protein 1; PRL, prolactin.
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Figure 3
Effects of silencing of SP1 and P300 on decidualization. 24 h after transfection with SP1 or P300 siRNA, hESCs were treated with db-cAMP and MPA for 4 days. (A and C) mRNA expression of related genes after SP1 was silenced. (B and D) mRNA expression of related genes after P300 was silenced. All data are shown as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001. IGFBP1, insulin-like growth factor binding protein 1; PRL, prolactin; VEGF, vascular endothelial growth factor.
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Figure 4
mRNA expression of SP1, P300, IGFBP1 and PRL in primary ESCs after induced decidualization. (A and B) mRNA levels of SP1 and P300 after induced decidualization for 6 days. (C and D) mRNA levels of IGFBP1 and PRL after induced decidualization at 0, 3 and 6 days. All data are shown as mean ± s.e.m. *P < 0.05; **P < 0.01 vs Ctrl or day 0. ## P < 0.01 vs day 3. Ctrl, control primary ESCs; cAMP + MPA, treated primary ESCs. IGFBP1, insulin-like growth factor-binding protein 1; PRL, prolactin.
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Figure 5
Effects of SP1 and P300 silencing on primary ESCs decidualization. (A) mRNA expression of related genes after SP1 was silenced. (B) mRNA expression of related genes after P300 was silenced. All data are shown as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001. IGFBP1, insulin-like growth factor-binding protein 1; PRL, prolactin; VEGF, vascular endothelial growth factor.
- © 2018 Society for Endocrinology
