60 YEARS OF POMC: N-terminal POMC peptides and adrenal growth

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   Figure 1

   Stimulation of rat adrenal cells by various N-POMC peptides. Dispersed rat adrenal cells were incubated for 3 h before the addition of N-POMC(2–59), N-POMC(1–28), N-POMC(1–76) (pro-γ-MSH), or ACTH(1–24) at various concentrations. The cells were incubated for a further 3 h before the addition of 1 μCi of ³H-thymidine. After a further 6 h, the medium was aspirated and ³H-thymidine incorporation determined as a measure of DNA synthesis. Adapted by permission from Macmillan Publishers Ltd: Nature (Estivariz et al. 1982), copyright (1982).
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   Figure 2

   Identification of trypsin-like serine proteases expressed in the rat adrenal following unilateral adrenalectomy. Rats underwent either unilateral adrenalectomy or a sham operation and were sacrificed either 6 or 24h postoperation. The RNA from the remaining gland was extracted and used to synthesise cDNA that was subsequently used as a template in the PCR with degenerate oligonucleotides designed around the conserved residues of the active site of the trypsin family of enzymes. PCR products were labelled with ³³P during synthesis, separated by polyacrylamide gel electrophoresis and subsequently cloned and sequenced. Band 1 was identified as tissue plasminogen activator, band 2 as an unknown sequence that we named AsP, and band 3 as adipsin. Reprinted from Cell, Vol 105, Bicknell AB, Lomthaisong K, Woods RJ, Hutchinson EG, Bennett HP, Gladwell RT & Lowry PJ, Characterization of a serine protease that cleaves progamma-melanotropin at the adrenal to stimulate growth, Pages 903–912, Copyright (2001), with permission from Elsevier.

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