Endocrine-Related Cancer

The primary structures of human BRCA1 and BRCA2. BRCA1 consists of 1863 amino acids. The RING domain is in the N-terminus and partly overlaps with the BARD1-binding region. BRCA1 interacts with PALB2 via a coiled-coil region in the C-terminus. The BRCT motif is a phosphorylated protein-binding sequence, and this motif mediates the association of BRCA1 with Abraxas, CtIP and BACH1, which are all known factors in DSB repair. BRCA2 comprises 3418 amino acids. The PALB2 binding region is located in the amino (N) terminus. In the center, BRCA2 harbors eight BRC repeats, which constitute a binding region for RAD51 monomers. In the carboxy (C) terminus, a DNA-binding domain contains three OB folds and a region that interacts with DSS1. The C-terminal region of BRCA2 is also important for the formation of the RAD51 nucleoprotein filament and BRCA2 nuclear localization.