...and 2.5 mmolL-1 L-glutamine, supplemented with 1.125 gL-1 NaHCO3, insulin (66 116 nmolL ), hydrocortisone (10 nmolL ), 17-estradiol (10 nmolL ), transferrin (10 mgml ), selenite 117 (30 nmolL-1),
penicillin (100 UmL-1), streptomycin (100 mgmL-1), and 2% fetal bovine serum 118 (FBS) (Krug et al ~~~