Supplementary Data

• Supplementary Figure 1 - Effect of single or combination of stains on RNA integrity. (A) No stain (i.e., control); (B) Acridine Orange; (C) Alcoholic cresol violet; (D) Alcoholic cresol violet and Eosin Y combination; (E) Arcturus Paradise and RNAse inhibitor; (F) Arcturus Paradise; (G) Haematoxylin, Eosin Y and RNAse inhibitor; (H) Haematoxylin and Eosin Y combination. Acridine orange and alcoholic cresyl violet stains are most effective in preserving human placental syncytiotrophoblast RNA. (PDF 356 KB)
• Supplementary Figure 2 - Effect of time taken on laser micro-dissection of syncytiotrophoblast. (A) 0 minute (i.e., control), (B) 30 minutes and (C) 50 minutes spent on laser-capture dissection. Degradation is proportional to the time spent on the dissection and (B) 30 minutes was considered to be optimal to allow laser-capture to be performed whilst maintaining RNA integrity. (PDF 198 KB)
• Supplementary Table 1 - Differentially expressed genes identified from each pairwise comparison (Contrast 1–3). (PDF 12 KB)
• Supplementary Table 2 - Probe level information of differentially expressed genes. (PDF 350 KB)
• Supplementary Table 3 - Gene Ontology term enrichment analysis. High confidence GO terms were considered as those with at least 3 associated genes identified from our input list (bolded entries). Based on this criterion, significantly enriched GO terms for Contrast 1 and Contrast 2 were virtually identical and related to a wide range of processes including protein metabolic process, RNA processing, gene expression and translational processes with the exception of GO:0022904 (respiratory electron transport chain) which was associated with Contrast 1 but not Contrast 2. In contrast, significantly enriched GO terms for Contrast 3 are distinct from those of Contrast 1 and 2; enriched GO terms annotated to RNA splicing processes, intracellular transport of proteins to Golgi. (PDF 291 KB)
• Supplementary Table 4 - KEGG pathway enrichment. High confidence KEGG terms were considered as those with at least 3 associated genes identified from our input list (bolded entries). Based on this criterion, several KEGG pathways including oxidative phosphorylation (Kegg:00190) and ribosome (Kegg:03010) pathways were predicted as being significantly enriched in both Contrast 1 and Contrast 2. However, only the spliceosome pathway (Kegg:03040) was found to be significantly over-represented in Contrast 3. (PDF 116 KB)
• Supplementary Table 5 - miRNA enrichment. Three miRNAs (hsa-miR-876-5p, hsa-miR-151-3p, hsa-miR-641) were predicted as being significantly overrepresented in Contrast 3 whilst no miRNA enrichment was found for Contrast 1 and 2. (PDF 181 KB)
• Supplementary Table 6 - qRT-PCR validation data of microarray. Four genes (PSG6; UBE2D3 T5 and T6 ; UBE2V1) were selected from genes that were at least 2-fold up regulated in the GDM group compared to Ob and Norm groups. The data are expressed in fold increases. p values are displayed. (PDF 8 KB)