Figure 7
Resolution by ion-exchange chromatography of acetyl α-MSH and des-acetyl α-MSH extracted from the pars intermedia of Xenopus laevis adapted to black or white backgrounds. The peptides purified by gel exclusion on Sephadex G-50 and ion-exchange chromatography on SP Sephadex C-25 were located by RIA with MSH antibody after (A) 2 weeks adaptation to a daylight background and (B and C) after 2 and 4 weeks adaptation to a black background. Reproduced, with permission, from Maruthainar et al. (1992).
