Journal of Molecular Endocrinology

Mutant FOXL2 fails to cooperate with SMAD3 on a composite regulatory element. (A) Luciferase activity of the reporter promoter 3XGRAS-luc in COV434 cells. Reporter vector (400 ng) was co-transfected with 200 ng of each of FOXL2 WT or C134W, TGFBR1-CA, SMAD3 and SMAD7, or pcDNA3.1 control vector, as indicated. Error bars represent the s.d. of five replicates. Asterisks represent a Student's t-test P value <10⁻³ between the indicated condition and control condition. The # symbol indicates a Student's t-test P value <10⁻³ between the FOXL2 WT and mutant conditions. (B) Expression value of SMAD1–9 mRNAs in COV434 cells and KGN cells, determined from microarray data (GEO datasets GSE39890 from Rosario et al. (2012)). Error bars represent the s.d.s of three conditions. (C) Luciferase activity of the reporter promoter 3XGRAS-luc in KGN cells. Reporter vector (400 ng) was co-transfected with 300 ng of each of FOXL2 WT or C134W, TGFBR1-CA or pcDNA3.1 control vector, as indicated. Error bars represent the s.d. of five replicates. Asterisks represent a Student's t-test P value <10⁻³, between the indicated condition and control condition. The # symbol indicates a Student's t-test P value <10⁻³ between the FOXL2 WT and mutant conditions. Experiments were led as in L'Hôte et al. (2012) and Benayoun et al. (2013).