Journal of Molecular Endocrinology

CHOP-10-dependent effect. Effect of LPS (1000 ng/ml) on CHOP-10 mRNA expression in adipose tissues (A) and in cultured 3T3-L1 adipocytes (B). The protein level of CHOP-10 in the presence of 1000 ng/ml LPS for 24 h in 3T3-L1 adipocytes (C). Blockade of JNK phosphorylation by SP600125 (20 μM) markedly attenuated the decrement of CHOP-10 mRNA (D) and protein (E) in cultured 3T3-L1 adipocytes. Over-expression of CHOP-10 blocked the inhibitory effect of LPS on the resistin mRNA (F). mRNA levels of CHOP-10 and resistin are normalized to β-actin mRNA levels, and the results are mean±s.e.m. of three independent experiments and analyzed by one-way ANOVA. *P<0.05, **P<0.01 compared with PBS group; ^#P<0.05, ^##P<0.01 LPS+SP600125 group compared with LPS group or LPS+antisense CHOP-10 group compared with LPS+missense CHOP-10 group; n=6.