Tuulikki Seppänen-Laakso; Matej Orešič

Figure 1

Summary of methods for lipid analysis. (A) Traditionally, serum cholesterol and triacylglycerol levels have been measured. (B) High performance liquid chromatographic separation using normal-phase techniques and evaporative light-scattering detection enables the separation of major lipid classes. (C and D) Isolation of phospholipid fraction (PL) is performed on silica thin-layer chromatography (TLC) and the fatty acid composition of total serum or its PL fraction is determined by capillary gas chromatography (GC). (E) Two-dimensional GC coupled to time-of-flight mass spectrometry (GC×GC-TOF) is used for small molecule metabolomics and (F–G) liquid chromatography-mass spectrometry (LC-MS) is commonly used in lipidomics. (H) Data mining and processing methods are needed to analyse the data.

References

↵

Adachi J,
Asano M,
Yoshioka N,
Nushida H &
Ueno Y

2006 Analysis of phosphatidylcholine oxidation products in human plasma using quadrupole time-of-flight mass spectrometry. Kobe Journal of Medical Sciences 52 127–140.

↵

Bligh EG &
Dyer WJ

1959 A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37 911–917.

↵

Bodennec J,
Koul O,
Aguado I,
Brichon G,
Zwingelstein G &
Portoukalian J

2000 A procedure for fractionation of sphingolipid classes by solid-phase extraction on amnopropyl cartridges. Journal of Lipid Research 41 1524–1530.

↵

Brown AH

Lipodomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis,Methods in Enzymologyvol 4322007aSan Diego:Academic Press:

↵

Brown AH

Lipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007bSan Diego:Academic Press:

↵

Brown AH

Lipodomics and Bioactive Lipids: Lipids and Cell Signaling,Methods in Enzymologyvol 4342007cSan Diego:Academic Press:

↵

Fahy E,
Subramaniam S,
Brown HA,
Glass CK,
Merrill AH Jr.,
Murphy RC,
Raetz CR,
Russell DW,
Seyama Y,
Shaw W
et al.

2005 A comprehensive classification system for lipids. Journal of Lipid Research 46 839–862.

↵

Folch J,
Lees M &
Sloane Stanley GH

1957 A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226 497–509.

↵

Friedewald WT,
Levy RI &
Fredrickson DS

1972 Estimation of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clinical Chemistry 18 499–502.

↵

Goodacre R,
Vaidyanathan S,
Dinn WB,
Harrigan GG &
Kell DB

2004 Metabolomics by numbers: acquiring and understanding global metabolite data. Trends in Biotechnology 22 245–252.

↵

Van der Greef J,
Stroobant P &
Van der Heijden R

2004 The role of analytical sciences in medical systems biology. Current Opinion in Chemical Biology 8 559–565.

↵

Han X &
Gross RW

2005 Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrometry Reviews 24 367–412.

↵

Holland WL,
Stauter EC &
Stith Bj

2003 Quantification of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light-scattering detection. Journal of Lipid Research 44 854–858.

Homan R &
Anderson MK

1998 Rapid separation and quantification of combined neutral and polar lipid classes by high-performance liquid chromatography and evaporative light-scattering mass detection. Journal of Chromatography. B 708 21–25.

↵

Houjou T,
Yamatani K,
Imagawa M,
Shimizu T &
Taguchi R

2005 A shotgun tandem mass spectrometric analysis of phospholipids with normal-phase and/or reverse-phase liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry 19 654–666.

↵

Jiye A,
Trygg J,
Gullberg J,
Johansson AI,
Jonsson P,
Antti H,
Marklund SL &
Moritz T

2005 Extraction and GC/MS analysis of the human blood plasma metabolome. Analytical Chemistry 77 8086–8094.

Katajamaa M &
Orešič M

2007 Data processing for mass spectrometry-based metabolomics. Journal of Chromatography. A 1158 318–328.

Khaselev N &
Murphy RC

2000 Structural characterization of oxidized phospholipid products derived from arachidonate-containing plasmenyl glycerophosphocholine. Journal of Lipid Research 41 564–572.

Koivusalo M,
Haimi P,
Heikinheimo L,
Kostiainen R &
Somerharju P

2001 Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response. Journal of Lipid Research 42 663–672.

↵

Laaksonen R,
Katajamaa M,
Päivä H,
Sysi-Aho M,
Saarinen L,
Junni P,
Lütjohann D,
Smet J,
Coster RV,
Seppänen-Laakso T
et al.

2006 A systems biology strategy reveals biological pathways and plasma biomarker candidates for potentially toxic statin-induced changes in muscle. PLoS ONE 1 e97

↵

Lutzke BS &
Braughler JM

1990 An improved method for the identification and quantitation of biological lipids by HPLC using laser light-scattering detection. Journal of Lipid Research 11 2127–2130.

↵

Manini P,
Andreoli R,
Careri M,
Elviri L &
Musci M

1998 Atmospheric pressure chemical ionization liquid chromatography/mass spectrometry in cholesterol oxide determination and characterization. Rapid Communications in Mass Spectrometry 12 883–889.

↵

Masoodi M &
Nicolaou A

2006 Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry 20 3023–3029.

↵

Masoodi M,
Mir AA,
Petasis NA,
Serhan CN &
Nicolau A

2008 Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry 22 75–83.

Medina-Gomez G,
Gray SL,
Yetukuri L,
Shimomura K,
Virtue S,
Campbell M,
Curtis RK,
Jimenez-Linan M,
Blount M,
Yeo GS
et al.

2007 PPAR gamma 2 prevents lipotoxicity by controlling adipose tissue expandability and peripheral lipid metabolism. PLoS Genetics 27 e64

Brown AH

Moore JD,
Caufield WV &
Shaw WA

Quantitation and standardization of lipid internal standards for mass spectroscopyBrown AHLipodomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis,Methods in Enzymologyvol 4322007San Diego:Academic Press:351–367.

Brown AH

Murph M,
Tanaka T,
Pang J,
Felix E,
Liu S,
Trost R,
Godwin AK,
Newman R &
Mills G

Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosisBrown AHLipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007San Diego:Academic Press:1–25.

Orešič M,
Hänninen V &
Vidal-Puig A

2008 Lipidomics: a new window to biomedical frontiers. Trends in Biotechnology 26 647–652.

↵

Schmitz G,
Liebisch G &
Langmann T

2006 Lipidomic strategies to study structural and functional defects of ABC-transporters in cellular lipid trafficking. FEBS Letters 580 5597–5610.

↵

Schwudke D,
Oegema J,
Burton L,
Entchev E,
Hannich JT,
Ejsing CS,
Kurzchalia T &
Shevchenko A

2006 Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition. Analytical Chemistry 78 585–595.

↵

Seppänen-Laakso T,
Laakso I,
Vanhanen H,
Kiviranta K,
Lehtimäki T &
Hiltunen R

2001 Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids. Journal of Chromatography. B 754 437–445.

↵

Seppänen-Laakso T,
Laakso I &
Hiltunen R

2002 Analysis of fatty acids by gas chromatography, and its relevance to research on health and nutrition. Analytica Chimica Acta 465 39–64.

↵

Sommer U,
Herscovitz H,
Welty FK &
Costello CE

2006 LC-MS based method for the qualitative and quantitative analysis of complex lipid mixtures. Journal of Lipid Research 47 804–814.

↵

Taguchi R,
Houjou T,
Nakanishi H,
Yamazaki T,
Ishida M,
Imagawa M &
Shimizu T

2005 Focused lipidomics by tandem mass spectrometry. Journal of Chromatography. B 823 26–36.

↵

von Thiele OW

Lipide, Isoprenoide mit Steroiden,1979Stuttgart:Georg Thieme Verlag:

↵

Unger RH

2002 Lipotoxic diseases. Annual Review of Medicine 53 319–336.

↵

Brown AH

Wang D &
DuBois RN

Measurement of eicosanoids in cancer tissueBrown AHLipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007San Diego:Academic Press:27–50.

↵

Want EJ,
Cravatt BF &
Siuzdak G

2005 The expanding role of mass spectrometry in metabolite profiling and characterization. Chembiochem 6 1941–1951.

↵

Watson AD

2006 A global approach to lipid analysis in biological systems. Journal of Lipid Research 47 2101–2111.

Yetukuri L,
Katajamaa M,
Medina-Gomez G,
Seppänen-Laakso T,
Vidal-Puig A &
Oresic M

2007 Bioinformatics strategies for lipidomics analysis: characterization of obesity related hepatic steatosis. BMC Systems Biology 1 12

Yetukuri L,
Ekroos K,
Vidal-Puig A &
Oresic M

2008 Informatics and computational strategies for the study of lipids. Molecular BioSystems 4 121–127.

↵

Zemski Berry KA &
Murphy RC

2005 Free radical oxidation of plasmalogen glycerophosphocholine containing esterified docosahexaenoic acid: structure determination by mass spectrometry. Antioxidants and Redox Signaling 7 157–169.

This Article

Published online before print December 5, 2008, doi: 10.1677/JME-08-0150 J Mol Endocrinol March 1, 2009 vol. 42 no. 3 185-190

[1] ↵

Adachi J,

Asano M,

Yoshioka N,

Nushida H &

Ueno Y

2006 Analysis of phosphatidylcholine oxidation products in human plasma using quadrupole time-of-flight mass spectrometry. Kobe Journal of Medical Sciences 52 127–140.

[2] Adachi J,

[3] Asano M,

[4] Yoshioka N,

[5] Nushida H &

[6] Ueno Y

[7] ↵

Bligh EG &

Dyer WJ

1959 A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37 911–917.

[8] Bligh EG &

[9] Dyer WJ

[10] ↵

Bodennec J,

Koul O,

Aguado I,

Brichon G,

Zwingelstein G &

Portoukalian J

2000 A procedure for fractionation of sphingolipid classes by solid-phase extraction on amnopropyl cartridges. Journal of Lipid Research 41 1524–1530.

[11] Bodennec J,

[12] Koul O,

[13] Aguado I,

[14] Brichon G,

[15] Zwingelstein G &

[16] Portoukalian J

[17] ↵

Brown AH

Lipodomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis,Methods in Enzymologyvol 4322007aSan Diego:Academic Press:

[18] Brown AH

[19] ↵

Brown AH

Lipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007bSan Diego:Academic Press:

[20] Brown AH

[21] ↵

Brown AH

Lipodomics and Bioactive Lipids: Lipids and Cell Signaling,Methods in Enzymologyvol 4342007cSan Diego:Academic Press:

[22] Brown AH

[23] ↵

Fahy E,

Subramaniam S,

Brown HA,

Glass CK,

Merrill AH Jr.,

Murphy RC,

Raetz CR,

Russell DW,

Seyama Y,

Shaw W

et al.

2005 A comprehensive classification system for lipids. Journal of Lipid Research 46 839–862.

[24] Fahy E,

[25] Subramaniam S,

[26] Brown HA,

[27] Glass CK,

[28] Merrill AH Jr.,

[29] Murphy RC,

[30] Raetz CR,

[31] Russell DW,

[32] Seyama Y,

[33] Shaw W

[34] et al.

[35] ↵

Folch J,

Lees M &

Sloane Stanley GH

1957 A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226 497–509.

[36] Folch J,

[37] Lees M &

[38] Sloane Stanley GH

[39] ↵

Friedewald WT,

Levy RI &

Fredrickson DS

1972 Estimation of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clinical Chemistry 18 499–502.

[40] Friedewald WT,

[41] Levy RI &

[42] Fredrickson DS

[43] ↵

Goodacre R,

Vaidyanathan S,

Dinn WB,

Harrigan GG &

Kell DB

2004 Metabolomics by numbers: acquiring and understanding global metabolite data. Trends in Biotechnology 22 245–252.

[44] Goodacre R,

[45] Vaidyanathan S,

[46] Dinn WB,

[47] Harrigan GG &

[48] Kell DB

[49] ↵

Van der Greef J,

Stroobant P &

Van der Heijden R

2004 The role of analytical sciences in medical systems biology. Current Opinion in Chemical Biology 8 559–565.

[50] Van der Greef J,

[51] Stroobant P &

[52] Van der Heijden R

[53] ↵

Han X &

Gross RW

2005 Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrometry Reviews 24 367–412.

[54] Han X &

[55] Gross RW

[56] ↵

Holland WL,

Stauter EC &

Stith Bj

2003 Quantification of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light-scattering detection. Journal of Lipid Research 44 854–858.

[57] Holland WL,

[58] Stauter EC &

[59] Stith Bj

[60] Homan R &

Anderson MK

1998 Rapid separation and quantification of combined neutral and polar lipid classes by high-performance liquid chromatography and evaporative light-scattering mass detection. Journal of Chromatography. B 708 21–25.

[61] Homan R &

[62] Anderson MK

[63] ↵

Houjou T,

Yamatani K,

Imagawa M,

Shimizu T &

Taguchi R

2005 A shotgun tandem mass spectrometric analysis of phospholipids with normal-phase and/or reverse-phase liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry 19 654–666.

[64] Houjou T,

[65] Yamatani K,

[66] Imagawa M,

[67] Shimizu T &

[68] Taguchi R

[69] ↵

Jiye A,

Trygg J,

Gullberg J,

Johansson AI,

Jonsson P,

Antti H,

Marklund SL &

Moritz T

2005 Extraction and GC/MS analysis of the human blood plasma metabolome. Analytical Chemistry 77 8086–8094.

[70] Jiye A,

[71] Trygg J,

[72] Gullberg J,

[73] Johansson AI,

[74] Jonsson P,

[75] Antti H,

[76] Marklund SL &

[77] Moritz T

[78] Katajamaa M &

Orešič M

2007 Data processing for mass spectrometry-based metabolomics. Journal of Chromatography. A 1158 318–328.

[79] Katajamaa M &

[80] Orešič M

[81] Khaselev N &

Murphy RC

2000 Structural characterization of oxidized phospholipid products derived from arachidonate-containing plasmenyl glycerophosphocholine. Journal of Lipid Research 41 564–572.

[82] Khaselev N &

[83] Murphy RC

[84] Koivusalo M,

Haimi P,

Heikinheimo L,

Kostiainen R &

Somerharju P

2001 Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response. Journal of Lipid Research 42 663–672.

[85] Koivusalo M,

[86] Haimi P,

[87] Heikinheimo L,

[88] Kostiainen R &

[89] Somerharju P

[90] ↵

Laaksonen R,

Katajamaa M,

Päivä H,

Sysi-Aho M,

Saarinen L,

Junni P,

Lütjohann D,

Smet J,

Coster RV,

Seppänen-Laakso T

et al.

2006 A systems biology strategy reveals biological pathways and plasma biomarker candidates for potentially toxic statin-induced changes in muscle. PLoS ONE 1 e97

[91] Laaksonen R,

[92] Katajamaa M,

[93] Päivä H,

[94] Sysi-Aho M,

[95] Saarinen L,

[96] Junni P,

[97] Lütjohann D,

[98] Smet J,

[99] Coster RV,

[100] Seppänen-Laakso T

[101] et al.

[102] ↵

Lutzke BS &

Braughler JM

1990 An improved method for the identification and quantitation of biological lipids by HPLC using laser light-scattering detection. Journal of Lipid Research 11 2127–2130.

[103] Lutzke BS &

[104] Braughler JM

[105] ↵

Manini P,

Andreoli R,

Careri M,

Elviri L &

Musci M

1998 Atmospheric pressure chemical ionization liquid chromatography/mass spectrometry in cholesterol oxide determination and characterization. Rapid Communications in Mass Spectrometry 12 883–889.

[106] Manini P,

[107] Andreoli R,

[108] Careri M,

[109] Elviri L &

[110] Musci M

[111] ↵

Masoodi M &

Nicolaou A

2006 Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry 20 3023–3029.

[112] Masoodi M &

[113] Nicolaou A

[114] ↵

Masoodi M,

Mir AA,

Petasis NA,

Serhan CN &

Nicolau A

2008 Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry 22 75–83.

[115] Masoodi M,

[116] Mir AA,

[117] Petasis NA,

[118] Serhan CN &

[119] Nicolau A

[120] Medina-Gomez G,

Gray SL,

Yetukuri L,

Shimomura K,

Virtue S,

Campbell M,

Curtis RK,

Jimenez-Linan M,

Blount M,

Yeo GS

et al.

2007 PPAR gamma 2 prevents lipotoxicity by controlling adipose tissue expandability and peripheral lipid metabolism. PLoS Genetics 27 e64

[121] Medina-Gomez G,

[122] Gray SL,

[123] Yetukuri L,

[124] Shimomura K,

[125] Virtue S,

[126] Campbell M,

[127] Curtis RK,

[128] Jimenez-Linan M,

[129] Blount M,

[130] Yeo GS

[131] et al.

[132] Brown AH

Moore JD,

Caufield WV &

Shaw WA

Quantitation and standardization of lipid internal standards for mass spectroscopyBrown AHLipodomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis,Methods in Enzymologyvol 4322007San Diego:Academic Press:351–367.

[133] Brown AH

[134] Moore JD,

[135] Caufield WV &

[136] Shaw WA

[137] Brown AH

Murph M,

Tanaka T,

Pang J,

Felix E,

Liu S,

Trost R,

Godwin AK,

Newman R &

Mills G

Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosisBrown AHLipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007San Diego:Academic Press:1–25.

[138] Brown AH

[139] Murph M,

[140] Tanaka T,

[141] Pang J,

[142] Felix E,

[143] Liu S,

[144] Trost R,

[145] Godwin AK,

[146] Newman R &

[147] Mills G

[148] Orešič M,

Hänninen V &

Vidal-Puig A

2008 Lipidomics: a new window to biomedical frontiers. Trends in Biotechnology 26 647–652.

[149] Orešič M,

[150] Hänninen V &

[151] Vidal-Puig A

[152] ↵

Schmitz G,

Liebisch G &

Langmann T

2006 Lipidomic strategies to study structural and functional defects of ABC-transporters in cellular lipid trafficking. FEBS Letters 580 5597–5610.

[153] Schmitz G,

[154] Liebisch G &

[155] Langmann T

[156] ↵

Schwudke D,

Oegema J,

Burton L,

Entchev E,

Hannich JT,

Ejsing CS,

Kurzchalia T &

Shevchenko A

2006 Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition. Analytical Chemistry 78 585–595.

[157] Schwudke D,

[158] Oegema J,

[159] Burton L,

[160] Entchev E,

[161] Hannich JT,

[162] Ejsing CS,

[163] Kurzchalia T &

[164] Shevchenko A

[165] ↵

Seppänen-Laakso T,

Laakso I,

Vanhanen H,

Kiviranta K,

Lehtimäki T &

Hiltunen R

2001 Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids. Journal of Chromatography. B 754 437–445.

[166] Seppänen-Laakso T,

[167] Laakso I,

[168] Vanhanen H,

[169] Kiviranta K,

[170] Lehtimäki T &

[171] Hiltunen R

[172] ↵

Seppänen-Laakso T,

Laakso I &

Hiltunen R

2002 Analysis of fatty acids by gas chromatography, and its relevance to research on health and nutrition. Analytica Chimica Acta 465 39–64.

[173] Seppänen-Laakso T,

[174] Laakso I &

[175] Hiltunen R

[176] ↵

Sommer U,

Herscovitz H,

Welty FK &

Costello CE

2006 LC-MS based method for the qualitative and quantitative analysis of complex lipid mixtures. Journal of Lipid Research 47 804–814.

[177] Sommer U,

[178] Herscovitz H,

[179] Welty FK &

[180] Costello CE

[181] ↵

Taguchi R,

Houjou T,

Nakanishi H,

Yamazaki T,

Ishida M,

Imagawa M &

Shimizu T

2005 Focused lipidomics by tandem mass spectrometry. Journal of Chromatography. B 823 26–36.

[182] Taguchi R,

[183] Houjou T,

[184] Nakanishi H,

[185] Yamazaki T,

[186] Ishida M,

[187] Imagawa M &

[188] Shimizu T

[189] ↵

von Thiele OW

Lipide, Isoprenoide mit Steroiden,1979Stuttgart:Georg Thieme Verlag:

[190] von Thiele OW

[191] ↵

Unger RH

2002 Lipotoxic diseases. Annual Review of Medicine 53 319–336.

[192] Unger RH

[193] ↵

Brown AH

Wang D &

DuBois RN

Measurement of eicosanoids in cancer tissueBrown AHLipodomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease,Methods in Enzymologyvol 4332007San Diego:Academic Press:27–50.

[194] Brown AH

[195] Wang D &

[196] DuBois RN

[197] ↵

Want EJ,

Cravatt BF &

Siuzdak G

2005 The expanding role of mass spectrometry in metabolite profiling and characterization. Chembiochem 6 1941–1951.

[198] Want EJ,

[199] Cravatt BF &

[200] Siuzdak G

[201] ↵

Watson AD

2006 A global approach to lipid analysis in biological systems. Journal of Lipid Research 47 2101–2111.

[202] Watson AD

[203] Yetukuri L,

Katajamaa M,

Medina-Gomez G,

Seppänen-Laakso T,

Vidal-Puig A &

Oresic M

2007 Bioinformatics strategies for lipidomics analysis: characterization of obesity related hepatic steatosis. BMC Systems Biology 1 12

[204] Yetukuri L,

[205] Katajamaa M,

[206] Medina-Gomez G,

[207] Seppänen-Laakso T,

[208] Vidal-Puig A &

[209] Oresic M

[210] Yetukuri L,

Ekroos K,

Vidal-Puig A &

Oresic M

2008 Informatics and computational strategies for the study of lipids. Molecular BioSystems 4 121–127.

[211] Yetukuri L,

[212] Ekroos K,

[213] Vidal-Puig A &

[214] Oresic M

[215] ↵

Zemski Berry KA &

Murphy RC

2005 Free radical oxidation of plasmalogen glycerophosphocholine containing esterified docosahexaenoic acid: structure determination by mass spectrometry. Antioxidants and Redox Signaling 7 157–169.

[216] Zemski Berry KA &

[217] Murphy RC

Journal of Molecular Endocrinology

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