Natacha Driessens; Soetkin Versteyhe; Chiraz Ghaddhab; Agnès Burniat; Xavier De Deken; Jacqueline Van Sande; Jacques-Emile Dumont; Françoise Miot; Bernard Corvilain

Figure 2

DNA DSBs evaluation using phosphorylation of histone H2AX in PCCl3 cells an hour after irradiation (A and B) or addition of H₂O₂ in the medium (C and D). Phosphorylated (γH2AX) and total histones H2AX were detected with specific antibodies. (A) Western blot showing a dose-dependent effect of irradiation on H2AX phosphorylation. (B) Immunocytochemistry detecting phosphorylated histone H2AX foci (pink spots). (C) Western blot showing dose-dependent effect of H₂O₂ on H2AX phosphorylation. (D) Immunocytochemistry detecting phosphorylated histone H2AX foci (pink spots). DSBs, double-strand breaks.

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↵

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2004 The comet assay for DNA damage and repair: principles, applications, and limitations. Molecular Biotechnology 26 249–261.

↵

Contempre B,
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2004 Thiocyanate induces cell necrosis and fibrosis in selenium- and iodine-deficient rat thyroids: a potential experimental model for myxedematous endemic cretinism in central Africa. Endocrinology 145 994–1002.

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↵

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↵

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↵

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2002 Characterization of ThOX proteins as components of the thyroid H₂O₂-generating system. Experimental Cell Research 273 187–196.

↵

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Delys L,
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Lacroix L,
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Maier J,
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2003 Biological chemistry of reactive oxygen and nitrogen and radiation-induced signal transduction mechanisms. Oncogene 22 5734–5754.

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Olive PL,
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1990 Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the ‘comet’ assay. Radiation Research 122 86–94.

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Pero RW,
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Picariello L,
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↵

Rigutto S,
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Remy C,
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This Article

Published online before print June 9, 2009, doi: 10.1677/ERC-09-0020 Endocr Relat Cancer September 1, 2009 vol. 16 no. 3 845-856

[1] ↵

Benard B &

Brault J

1971 Production of peroxide in the thyroid. L'Unión Médicale du Canada 100 701–705.

[2] Benard B &

[3] Brault J

[4] ↵

Bertram JS,

Kolonel LN &

Meyskens FL Jr.

1987 Rationale and strategies for chemoprevention of cancer in humans. Cancer Research 47 3012–3031.

[5] Bertram JS,

[6] Kolonel LN &

[7] Meyskens FL Jr.

[8] ↵

Bjorkman U &

Ekholm R

1988 Accelerated exocytosis and H₂O₂ generation in isolated thyroid follicles enhance protein iodination. Endocrinology 122 488–494.

[9] Bjorkman U &

[10] Ekholm R

[11] ↵

Bradley MO &

Kohn KW

1979 X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Research 7 793–804.

[12] Bradley MO &

[13] Kohn KW

[14] ↵

Chang CL,

Marra G,

Chauhan DP,

Ha HT,

Chang DK,

Ricciardiello L,

Randolph A,

Carethers JM &

Boland CR

2002 Oxidative stress inactivates the human DNA mismatch repair system. American Journal of Physiology. Cell Physiology 283 C148–C154.

[15] Chang CL,

[16] Marra G,

[17] Chauhan DP,

[18] Ha HT,

[19] Chang DK,

[20] Ricciardiello L,

[21] Randolph A,

[22] Carethers JM &

[23] Boland CR

[24] ↵

Chico Galdo V,

Massart C,

Jin L,

Vanvooren V,

Caillet-Fauquet P,

Andry G,

Lothaire P,

Dequanter D,

Friedman M &

Van Sande J

2006 Acrylamide, an in vivo thyroid carcinogenic agent, induces DNA damage in rat thyroid cell lines and primary cultures. Molecular and Cellular Endocrinology 257–258 6–14.

[25] Chico Galdo V,

[26] Massart C,

[27] Jin L,

[28] Vanvooren V,

[29] Caillet-Fauquet P,

[30] Andry G,

[31] Lothaire P,

[32] Dequanter D,

[33] Friedman M &

[34] Van Sande J

[35] ↵

Coclet J,

Foureau F,

Ketelbant P,

Galand P &

Dumont JE

1989 Cell population kinetics in dog and human adult thyroid. Clinical Endocrinology 31 655–665.

[36] Coclet J,

[37] Foureau F,

[38] Ketelbant P,

[39] Galand P &

[40] Dumont JE

[41] ↵

Collins AR

2004 The comet assay for DNA damage and repair: principles, applications, and limitations. Molecular Biotechnology 26 249–261.

[42] Collins AR

[43] ↵

Contempre B,

de Escobar GM,

Denef JF,

Dumont JE &

Many MC

2004 Thiocyanate induces cell necrosis and fibrosis in selenium- and iodine-deficient rat thyroids: a potential experimental model for myxedematous endemic cretinism in central Africa. Endocrinology 145 994–1002.

[44] Contempre B,

[45] de Escobar GM,

[46] Denef JF,

[47] Dumont JE &

[48] Many MC

[49] ↵

Corvilain B,

Laurent E,

Lecomte M,

Vansande J &

Dumont JE

1994 Role of the cyclic adenosine 3′,5′-monophosphate and the phosphatidylinositol-Ca²⁺ cascades in mediating the effects of thyrotropin and iodide on hormone synthesis and secretion in human thyroid slices. Journal of Clinical Endocrinology and Metabolism 79 152–159.

[50] Corvilain B,

[51] Laurent E,

[52] Lecomte M,

[53] Vansande J &

[54] Dumont JE

[55] ↵

Corvilain B,

Collyn L,

van Sande J &

Dumont JE

2000 Stimulation by iodide of H₂O₂ generation in thyroid slices from several species. American Journal of Physiology. Endocrinology and Metabolism 278 E692–E699.

[56] Corvilain B,

[57] Collyn L,

[58] van Sande J &

[59] Dumont JE

[60] ↵

De Deken X,

Wang D,

Many MC,

Costagliola S,

Libert F,

Vassart G,

Dumont JE &

Miot F

2000 Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family. Journal of Biological Chemistry 275 23227–23233.

[61] De Deken X,

[62] Wang D,

[63] Many MC,

[64] Costagliola S,

[65] Libert F,

[66] Vassart G,

[67] Dumont JE &

[68] Miot F

[69] ↵

De Deken X,

Wang D,

Dumont JE &

Miot F

2002 Characterization of ThOX proteins as components of the thyroid H₂O₂-generating system. Experimental Cell Research 273 187–196.

[70] De Deken X,

[71] Wang D,

[72] Dumont JE &

[73] Miot F

[74] ↵

Detours V,

Delys L,

Libert F,

Weiss SD,

Bogdanova T,

Dumont JE,

Franc B,

Thomas G &

Maenhaut C

2007 Genome-wide gene expression profiling suggests distinct radiation susceptibilities in sporadic and post-Chernobyl papillary thyroid cancers. British Journal of Cancer 97 818–825.

[75] Detours V,

[76] Delys L,

[77] Libert F,

[78] Weiss SD,

[79] Bogdanova T,

[80] Dumont JE,

[81] Franc B,

[82] Thomas G &

[83] Maenhaut C

[84] ↵

Duntas LH

2006 The role of selenium in thyroid autoimmunity and cancer. Thyroid 16 455–460.

[85] Duntas LH

[86] ↵

Dupuy C,

Ohayon R,

Valent A,

Noel-Hudson MS,

Deme D &

Virion A

1999 Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cdnas. Journal of Biological Chemistry 274 37265–37269.

[87] Dupuy C,

[88] Ohayon R,

[89] Valent A,

[90] Noel-Hudson MS,

[91] Deme D &

[92] Virion A

[93] ↵

Ip C,

Lisk DJ &

Scimeca JA

1994 Potential of food modification in cancer prevention. Cancer Research 54 1957s–1959s.

[94] Ip C,

[95] Lisk DJ &

[96] Scimeca JA

[97] ↵

Kaprara A &

Krassas GE

2006 Selenium and thyroidal function; the role of immunoassays. Hellenic Journal of Nuclear Medicine 9 195–203.

[98] Kaprara A &

[99] Krassas GE

[100] ↵

Kim H,

Lee TH,

Park ES,

Suh JM,

Park SJ,

Chung HK,

Kwon OY,

Kim YK,

Ro HK &

Shong M

2000 Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide-induced apoptosis in thyroid cells. Journal of Biological Chemistry 275 18266–18270.

[101] Kim H,

[102] Lee TH,

[103] Park ES,

[104] Suh JM,

[105] Park SJ,

[106] Chung HK,

[107] Kwon OY,

[108] Kim YK,

[109] Ro HK &

[110] Shong M

[111] ↵

Kohrle J,

Jakob F,

Contempre B &

Dumont JE

2005 Selenium, the thyroid, and the endocrine system. Endocrine Reviews 26 944–984.

[112] Kohrle J,

[113] Jakob F,

[114] Contempre B &

[115] Dumont JE

[116] ↵

Krohn K &

Paschke R

2002 Somatic mutations in thyroid nodular disease. Molecular Genetics and Metabolism 75 202–208.

[117] Krohn K &

[118] Paschke R

[119] ↵

Lacroix L,

Soria JC,

Bidart JM &

Schlumberger M

2005 Oncogenes and thyroid tumors. Bulletin du Cancer 92 37–43.

[120] Lacroix L,

[121] Soria JC,

[122] Bidart JM &

[123] Schlumberger M

[124] ↵

Maier J,

van Steeg H,

van Oostrom C,

Karger S,

Paschke R &

Krohn K

2006 Deoxyribonucleic acid damage and spontaneous mutagenesis in the thyroid gland of rats and mice. Endocrinology 147 3391–3397.

[125] Maier J,

[126] van Steeg H,

[127] van Oostrom C,

[128] Karger S,

[129] Paschke R &

[130] Krohn K

[131] ↵

Mikkelsen RB &

Wardman P

2003 Biological chemistry of reactive oxygen and nitrogen and radiation-induced signal transduction mechanisms. Oncogene 22 5734–5754.

[132] Mikkelsen RB &

[133] Wardman P

[134] ↵

Nasir A,

Chaudhry AZ,

Gillespie J &

Kaiser HE

2000 Papillary microcarcinoma of the thyroid: a clinico-pathologic and prognostic review. In Vivo 14 367–376.

[135] Nasir A,

[136] Chaudhry AZ,

[137] Gillespie J &

[138] Kaiser HE

[139] ↵

Neumann CA,

Krause DS,

Carman CV,

Das S,

Dubey DP,

Abraham JL,

Bronson RT,

Fujiwara Y,

Orkin SH &

Van Etten RA

2003 Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 424 561–565.

[140] Neumann CA,

[141] Krause DS,

[142] Carman CV,

[143] Das S,

[144] Dubey DP,

[145] Abraham JL,

[146] Bronson RT,

[147] Fujiwara Y,

[148] Orkin SH &

[149] Van Etten RA

[150] ↵

Nunez J &

Pommier J

1982 Formation of thyroid hormones. Vitamins and Hormones 39 175–229.

[151] Nunez J &

[152] Pommier J

[153] ↵

Olive PL,

Banath JP &

Durand RE

1990 Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the ‘comet’ assay. Radiation Research 122 86–94.

[154] Olive PL,

[155] Banath JP &

[156] Durand RE

[157] ↵

Pero RW,

Anderson MW,

Doyle GA,

Anna CH,

Romagna F,

Markowitz M &

Bryngelsson C

1990 Oxidative stress induces DNA damage and inhibits the repair of DNA lesions induced by N-acetoxy-2-acetylaminofluorene in human peripheral mononuclear leukocytes. Cancer Research 50 4619–4625.

[158] Pero RW,

[159] Anderson MW,

[160] Doyle GA,

[161] Anna CH,

[162] Romagna F,

[163] Markowitz M &

[164] Bryngelsson C

[165] ↵

Picariello L,

Sala SC,

Martineti V,

Gozzini A,

Aragona P,

Tognarini I,

Paglierani M,

Nesi G,

Brandi ML &

Tonelli F

2006 A comparison of methods for the analysis of low abundance proteins in desmoid tumor cells. Analytical Biochemistry 354 205–212.

[166] Picariello L,

[167] Sala SC,

[168] Martineti V,

[169] Gozzini A,

[170] Aragona P,

[171] Tognarini I,

[172] Paglierani M,

[173] Nesi G,

[174] Brandi ML &

[175] Tonelli F

[176] ↵

Rigutto S,

Hoste C,

Dumont JE,

Corvilain B,

Miot F &

De Deken X

2007 Duox1 is the main source of hydrogen peroxide in the rat thyroid cell line PCCl3. Experimental Cell Research 313 3892–3901.

[177] Rigutto S,

[178] Hoste C,

[179] Dumont JE,

[180] Corvilain B,

[181] Miot F &

[182] De Deken X

[183] ↵

Riou C,

Remy C,

Rabilloud R,

Rousset B &

Fonlupt P

1998 H₂O₂ induces apoptosis of pig thyrocytes in culture. Journal of Endocrinology 156 315–322.

[184] Riou C,

[185] Remy C,

[186] Rabilloud R,

[187] Rousset B &

[188] Fonlupt P

[189] ↵

Riou C,

Tonoli H,

Bernier-Valentin F,

Rabilloud R,

Fonlupt P &

Rousset B

1999 Susceptibility of differentiated thyrocytes in primary culture to undergo apoptosis after exposure to hydrogen peroxide: relation with the level of expression of apoptosis regulatory proteins, Bcl-2 and Bax. Endocrinology 140 1990–1997.

[190] Riou C,

[191] Tonoli H,

[192] Bernier-Valentin F,

[193] Rabilloud R,

[194] Fonlupt P &

[195] Rousset B

[196] ↵

Rogakou EP,

Pilch DR,

Orr AH,

Ivanova VS &

Bonner WM

1998 DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. Journal of Biological Chemistry 273 5858–5868.

[197] Rogakou EP,

[198] Pilch DR,

[199] Orr AH,

[200] Ivanova VS &

[201] Bonner WM

[202] ↵

Roger P,

Taton M,

Van Sande J &

Dumont JE

1988 Mitogenic effects of thyrotropin and adenosine 3′,5′-monophosphate in differentiated normal human thyroid cells in vitro. Journal of Clinical Endocrinology and Metabolism 66 1158–1165.

[203] Roger P,

[204] Taton M,

[205] Van Sande J &

[206] Dumont JE

[207] ↵

Ron E,

Lubin JH,

Shore RE,

Mabuchi K,

Modan B,

Pottern LM,

Schneider AB,

Tucker MA &

Boice JD Jr.

1995 Thyroid cancer after exposure to external radiation: a pooled analysis of seven studies. Radiation Research 141 259–277.

[208] Ron E,

[209] Lubin JH,

[210] Shore RE,

[211] Mabuchi K,

[212] Modan B,

[213] Pottern LM,

[214] Schneider AB,

[215] Tucker MA &

[216] Boice JD Jr.

[217] ↵

Ross DS

2002 Nonpalpable thyroid nodules – managing an epidemic. Journal of Clinical Endocrinology and Metabolism 87 1938–1940.

[218] Ross DS

[219] ↵

Sarasin A,

Bounacer A,

Lepage F,

Schlumberger M &

Suarez HG

1999 Mechanisms of mutagenesis in mammalian cells. Application to human thyroid tumours. Comptes Rendus de l'Académie des Sciences. Série III 322 143–149.

[220] Sarasin A,

[221] Bounacer A,

[222] Lepage F,

[223] Schlumberger M &

[224] Suarez HG

[225] ↵

Singh NP,

McCoy MT,

Tice RR &

Schneider EL

1988 A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175 184–191.

[226] Singh NP,

[227] McCoy MT,

[228] Tice RR &

[229] Schneider EL

[230] ↵

Song Y,

Driessens N,

Costa M,

De Deken X,

Detours V,

Corvilain B,

Maenhaut C,

Miot F,

van Sande J,

Many M-C

et al.

2007 Roles of hydrogen peroxide in thyroid physiology and disease. Journal of Clinical Endocrinology and Metabolism 92 3764–3773.

[231] Song Y,

[232] Driessens N,

[233] Costa M,

[234] De Deken X,

[235] Detours V,

[236] Corvilain B,

[237] Maenhaut C,

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Endocrine-Related Cancer

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