TY - JOUR A1 - Umpleby, A Margot T1 - HORMONE MEASUREMENT GUIDELINES: Tracing lipid metabolism: the value of stable isotopes Y1 - 2015/09/01 JO - Journal of Endocrinology SP - G1 EP - G10 DO - 10.1530/JOE-14-0610 N1 - 10.1530/JOE-14-0610 M3 - 10.1530/JOE-14-0610 VL - 226 IS - 3 UR - http://joe.endocrinology-journals.org/content/226/3/G1.abstract N2 - Labelling molecules with stable isotopes to create tracers has become a gold-standard method to study the metabolism of lipids and lipoproteins in humans. There are a range of techniques which use stable isotopes to measure fatty acid flux and oxidation, hepatic fatty synthesis, cholesterol absorption and synthesis and lipoprotein metabolism in humans. Stable isotope tracers are safe to use, enabling repeated studies to be undertaken and allowing studies to be undertaken in children and pregnant women. This review provides details of the most appropriate tracers to use, the techniques which have been developed and validated for measuring different aspects of lipid metabolism and some of the limitations of the methodology. ER -